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Keywords 16S rRNA gene;pulsed field gel electrophoresis;streptococcus mitis; 16S核糖体RNA基因;脉冲场凝胶电泳;缓症链球菌;
Keywords 16S rRNA;gene chip;diagnose;bacteria; 基因芯片;诊断;细菌;
Nucleotide sequences of 472~481bp for 16S rRNA gene and 658bp for COI gene were obtained, respectively. 得到的序列总长度分别为472~481bp(16S)和658bp(COI)。
In this study, phylogenetic relationship of 7 Soleoidei species are analysed by sequence variation of both 16S rRNA gene and COI gene. 本文对7种鳎亚目(Soleoidei)鱼类线粒体16S rRNA基因片段和COI基因片段进行了序列比较和分子系统学分析。
Genetic distances between S. grandis and S. strictus were 0.0856 (with 16S rRNA gene) and 0.1712 (with COI gene), respectively. 数据分析结果表明16S rRNA和COI基因片段大竹蛏与长竹蛏两片段的遗传距离分别为0.;0856和0 1712;两竹蛏类与小刀蛏的遗传距离分别为0
Analysis of 16S rRNA gene sequence indicated that the alkaliphilic strain EMB2039 exhibited maximum similarity with the modal strain DSM 6307~T of Bacillus cohnii. 16 S rDNA测序及系统发育分析结果表明EMB2039与Bacillus cohnii模式菌株DSM 6307~T亲缘关系最近。
On the basis of homology and phylognetic analysis about 16S rRNA gene (16S rDNA), it could be speculated that the strain AB1 is a novel member of the genus Natronococcus. 基于16S rDNA序列的同源性比较及系统发育学研究表明，AB1是Natronococcus属中新成员。
Keywords Vibrio cholerae;Cholerae enterotoxin;16S rRNA gene typing; 霍乱弧菌;霍乱肠毒素;核糖体基因分型;
The genomic DNA was isolated and purified with the improved CTAB methods. The sequences of 16S rRNA gene were amplified by TD-PCR with the bacterium universal primers and the purified PCR products were directly sequenced. 菌株经纯化培养;以改良CTAB法提取总DNA;采用细菌16S rRNA通用引物、TD-PCR方法(touchdown-PCR)进行16S rRNA基因序列扩增;PCR产物纯化后直接进行序列测定;序列经人工校对后用Clustal X进行比对分析;最后用MEGA3.;1软件构建系统发育树。
To detect the variability and its pathogenecity of Helicobacter pylori(H.pylori),fifty- two biopsy specimens and 18 strains of H.pylori were performed PCR for 16S rRNA gene and flagellin A gene(flaA). PCR-RFLP and PCR-RFLP-SSCP were also performed. 我们对52例胃粘膜活检标本和18株 Hp 临床分离株进行 Hp 特异的16S rRNA 基因和鞭毛素 A 基因 PCR 扩增和 PCR-RFLP 及 PCR-RFLP-SSCP 分析,以检测 Hp 鞭毛素 A 基因的变异性,进而研究其与 Hp 致病性的关系。
Blanchard A, Yanez A, Dybvig K, et al.Evaluation of intraspecies genetic variation within the 16S rRNA gene mycoplasmas homini and detecting by polymerase chainreaction.J Clin Microbiol, 1993,31:1358. 骆丹;黄澍杰;谢礼豪;等.;解脲支原体对七种抗菌药物的敏感性测定及其与相关耐药基因检测的比较
Method Acoording to the analysis of the conservative and variable regions in bacterial 16S rRNA genes , we designed universal primers for all bacteria and specific primers for most gram-positive and gram-negtive bacteria . 方法通过对多数病原菌16S rRNA基因保守区和变异区的序列分析，设计通用引物和革兰阴性菌及革兰阳性菌的特异性引物分别作为外侧和内侧引物，建立了对不同细菌的16S rRNA基因进行多重半套式PCR扩增方法；
The genetic diversities of cytochrome b, COI, ND2, ND4, 12S rRNA and 16S rRNA genes of mtDNA were analyzed by PCR-SSCP method among these individuals, and variable sites were found through sequencing analysis which led to the diversities. 通过PCR-SSCP 及DNA 测序的方法对这些品种(共468 个体)线粒体DNA 编码区的Cytb、COI、ND2、ND4、12S rRNA 和16SrRNA 基因设计特异性引物进行多态性分析,并通过测序分析找出了导致多态的变异位点。
Study on COI and 16S rRNA gene sequences of the common Chinese cuttlefish Sepiella maindroni 曼氏无针乌贼Sepiella maindroni COI和16S rRNA基因序列研究
Sequence Analysis of 16s rRNA Gene from 3 Microcystis flos-aquaeaeruginosa in Guangdong Rerservoirs 广东省水库3株水华微囊藻16srRNA序列分析
aromatic hydrocarbons, by 16S rRNA gene sequncing and amplified. fragment length polymorphism fingerprinting. 陈宇炜;高锡云;陈伟民等.;太湖微囊藻的生长特征及其分离纯培养的初步研究
The cloned 16S rRNA gene sequence was 1435 nucleotides in length, and was highly homology to that of Bacillus subtillis reported in GenBank, with similanity of 100% to AY601722; 所克隆的16S rRNA序列全长1435bp，BLAST分析结果显示，该序列与GenBank中收录的枯草芽胞杆菌16S rRNA基因相似性最高，其中与AY601722的相似性为100%25；
16S rRNA gene 16SrRNA基因
16S rRNA gene clone library 16SrRNA基因
Mitochondrial 16S rRNA and COI gene fragments of Portunus trituberculatus from Weifang were amplified via PCR, then the PCR products were purified and sequenced. 本文采用 PCR扩增和序列测定等技术 ,对三疣梭子蟹 (Portunustrituberculatus)线粒体DNA1 6Sr RNA和 COI基因片段进行了初步研究。